Organizing Gold Nanoparticles with DNA

“The collective properties of nanoparticles are heavily dependent on how the particles are grouped. Achieving an even spacing between the particles is particularly important, but can be difficult,” said the study’s lead scientist, ASU chemist Hao Yan. “However, when deposited onto a DNA grid the particles fall neatly into patterns with little effort on our part.”

(a) The two-tile system that forms the DNA nanogrids. Tile A is blue and tile B is orange. The numbers indicate the complementary “sticky” ends that allow the tiles to adhere together, with 1 pairing with 1´ and so on. The red strand on tile A is A15. (b) The DNA nanogrid, showing the A₁₅ strand on each A tile. (c) Gold nanoparticles on the DNA grids. The zigzagged black lines surrounding the nanoparticles represent T₁₅ strands. Credit: Hao Yan

Yan and his research group used gold nanoparticles that were five nanometers in diameter. Rather than being bare, the particles were coated with a layer of DNA “pieces,” called “T₁₅ sequences,” which radiated from the particles’ surfaces like arms. The scientists then deposited the particles onto lattices formed by two types of cross-shaped DNA “tiles”, “A” tiles and “B” tiles, that bind together in an alternating fashion to form the DNA grid.

At regular intervals, each A tile contained a short single strand (called an “A₁₅” strand) that protruded out of the tile surface. These strands served as tethering points for the T₁₅-coated nanoparticles, allowing the particles to stick to the DNA surface, a bit like DNA-nanoparticle “Velcro.”

This configuration caused the nanoparticles to “self assemble” into a square pattern — each particle sitting on one A tile — with a nearly constant particle-particle distance of about 38 nanometers. The group confirmed this using an atomic force microscope, a very powerful imaging device.

However, this result, while welcomed by the scientists, wasn’t exactly what they expected.

“We were pleased that the gold nanoparticles formed a very regular square pattern, but it wasn’t quite the pattern we thought we’d see,” said Yan. “If you picture nine DNA tiles forming a square, we predicted that five particles would be organized on the square — one on each corner and one in the middle. But the pattern we observed lacked that middle particle.”

The scientists guess that this is due to the T₁₅ sequence layer, which effectively increases the diameter of each nanoparticle and, moreover, makes each particle highly negatively charged. As a result, the nanoparticles repel each other if they are too close together, which limits the minimum particle-particle distance. Therefore, a particle located at the center of the square would violate this limit.

In future research, Yan and may try to use this organization method to form more complex nanoparticle arrays, such as denser patterns or patterns of different shapes, by altering the particles’ DNA coating.

Citation: “Periodic Square-Like Gold Nanoparticle Arrays Templated by Self-Assembled 2D DNA Nanogrids on a Surface,” Nano Lett., Vol. 6, No. 2, 248-251 (2006)

by Laura Mgrdichian, Copyright 2006 PhysOrg.com