DNA Barcodes: Are They Always Accurate?

But the approach as currently practiced churns out some results as inaccurately as a supermarket checker scanning an apple and ringing it up as an orange, according to a new Brigham Young University (BYU) study.

The results are published online this week in the journal Proceedings of the National Academy of Sciences (PNAS). The researchers recommend specific quality control procedures to ensure that correct genes are captured.

"It's important to test any scientific tool because all have limits--some situations are more suited than others for barcode use," said Rick McCourt, program director in the National Science Foundation (NSF)'s Division of Environmental Biology, which funded the work. "This research could help clarify the answer to that question."

Organisms can be identified no matter what stage of life they are in. For example, larvae of malaria-carrying mosquitoes contain the same DNA as the adult version of the insect targeted for eradication. The portion of the gene selected as the universal marker by the barcoding movement is part of the genome found in an organism's mitochondria. But the BYU study showed the current techniques can mistakenly record instead the "broken" copy of the gene found in the nucleus of the organism's cells.

This non-functional copy can be similar enough for the barcoding technique to capture, but different enough to call it a unique species, which would be a mistake.

With the International Barcode of Life project seeking to build on the 400,000 species that have been "barcoded" to date, this goal warrants more careful execution, the BYU team says.

"To have that kind of data is hugely valuable, and the list of applications is endless and spans all of biology," said PNAS paper co-author Keith Crandall, a biologist at BYU. "But it all hinges on building an accurate database. Our study is a cautionary tale--if we're going to do it, let's do it right."

Proponents of DNA barcoding seek to establish a short genetic sequence as a way of identifying species in addition to traditional approaches based on external physical features.

Their aim is to create a giant library full of these sequences. Scientists foresee a future handheld device like a supermarket scanner--a machine that would sequence a DNA marker from an organism, then compare it with the known encyclopedia of life and spit out the species' name.

This new approach requires only part of a sample. A feather left behind by a bird struck by an airliner, for example, would be enough to indicate its species and clue officials how to prevent future collisions.

"Building a genetic library of all life is a great goal," said Song, "but we need to pay careful attention to the data that go into that library to make sure they are accurate."

"Scientists have been so preoccupied with creating a barcode of life, that they have not been careful in monitoring the accuracy of the underlying data," BYU scientist Michael Whiting said.

Provided by NSF