Bacteria detoxify deadly seawater

December 11, 2008

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Just the tip of the iceberg? A large patch of discolored surface waters off Namibia attributed to toxic hydrogen sulphide release from the seafloor. Many of these naturally occurring events may go unnoticed by satellite because bacteria consume sulphide before it reaches the surface. Image: Jacques Descloitres, MODIS Rapid Response Team, NASA/GSFC

(PhysOrg.com) -- Some marine bacteria produce hydrogen sulphide, which is toxic to animals. Scientists have now discovered that bacteria also protect marine animals from this toxic gas. A bacterial bloom detoxified a vast expanse of hydrogen sulphide-containing water off the coast of Namibia, before it could unfold its full deadly impact. (Nature, early online edition, December 11th, 2008)

Hydrogen sulphide (H₂S) is well known for its characteristic smell of rotten eggs. But hydrogen sulphide is not only smelly, it is also highly toxic. Humans can die within minutes when exposed to high concentrations of hydrogen sulphide. This foul-smelling gas threatens coastal fisheries, which account for 90 percent of global fish-catch. Eutrophication of coastal waters results in the episodic development of sulphidic water masses, with disastrous consequences for coastal ecosystems.

Bacteria play a dubious role in the process - after all, they are responsible for the formation of deadly hydrogen sulphide gas. However, bacteria are also responsible for detoxifying hydrogen sulphide, as researchers from the German Max-Planck-Institute for Marine Microbiology in Bremen, the Namibian National Marine Information & Research Centre, the German Baltic Sea Research Institute Warnemünde and the Department of Microbial Ecology of the University of Vienna now discovered. Their surprising results show that bacteria detoxified sulphidic coastal waters covering an area of approximately 7,000 km2 off Namibia - an area almost three times the size of Luxembourg.

The scientists investigated the occurrence of sulphidic water masses along the coast of West Africa. In January 2004, they hit upon a sulphidic water mass covering 7,000 km2 of coastal seafloor. The surface waters, however, were well oxygenated. In the presence of oxygen, sulphide is oxidized and transformed into nontoxic forms of sulphur. Surprisingly, Lavik, Stührmann, Kuypers and their colleagues found an intermediate layer in the water column, which contained neither hydrogen sulphide nor oxygen. What had happened to the poisonous gas?

"Obviously it was oxidized anaerobically - without oxygen", Torben Stührmann explains. "Many bacteria do not require oxygen for respiration and can use nitrate (NO₃^-) instead. And indeed - we found a water layer that contained both hydrogen sulphide and nitrate."

This water layer is the habitat of detoxifying microorganisms, which are closely related to bacteria known from hot vents and cold seeps in the deep ocean. By using nitrate, these bacteria transform sulphide into finely dispersed particles of sulphur that are nontoxic. Thus, the microorganisms create a buffer zone between the toxic deep water and the oxygenated surface waters, where fish and other marine animals live.

These results, published in the journal "Nature", are not only relevant for West African fisheries. They also suggest that animals living on or near the seafloor in coastal waters all over globe may be affected by such toxic water masses more often than previously thought. Up to now, sulphidic water masses were monitored with the help of satellites, taking pictures of the sea surface while orbiting the earth. Sulphur shows up in satellite pictures as whitish/turquoise discolorations of surface water. However, many of these sulphidic events may go unnoticed by satellite because bacteria consume the hydrogen sulphide before it reaches the surface. "We assume there are many more of these sulphidic events than previously thought", Namibian marine scientist Anja van der Plas comments. "Yet, they go unnoticed using conventional methods."

"There is a very positive as well as a worrying aspect of our discovery of a gigantic bacterial bloom detoxifying hydrogen sulphide", summarizes group leader Marcel Kuypers, "Hydrogen sulphide is toxic to higher life and even at low concentrations it will instantly kill fish, oysters, shrimps and lobsters. The good news is that the discovered groups of bacteria seem to consume the hydrogen sulphide before it reaches the surface waters where fish are living. It is worrying news, however, that an area the size of the Irish Sea or the Wadden Sea was affected by sulphidic bottom waters, without this being visible on satellite photos or detected at the monitoring stations closer to the coast."

Mass death by suffocation in coastal waters is not restricted to the coast off southwest Africa, where sulphidic events occur naturally. Such occurrences have also been reported for coastal waters off India, California, the Gulf of Mexico as well as European coastal waters. "There is increasing evidence demonstrating that global warming and high anthropogenic nutrient load to coastal waters are causing more frequent coastal anoxia, which in turn increases the risk of sulphide in coastal waters", Gaute Lavik explains. "However, the fact that we can correlate sulphidic waters with certain environmental conditions might provide an opportunity to forecast these events in the future."

Background: Toxic hydrogen sulphide

Hydrogen sulphide develops in anoxic environments where sulphate respiring bacteria - so-called sulphate reducers - degrade animal or plant remains. The conspicuous smell of rotten eggs is a striking warning that this toxic gas is released. At first, the gas irritates human eyes and respiratory tracts and higher concentrations result in death by apnoea within seconds.

Some scientists think that hydrogen sulphide is responsible for mass extinctions of numerous animal and plant species in the early history of the Earth. They argue that declining oxygen concentrations in the oceans could have caused hydrogen sulphide to rise from deeper water layers. Subsequently, the toxic gas was released into the atmosphere, unfolding its deadly impact on the land ecosystem.

More information: Van der Plas, Volker Mohrholz, Phyllis Lam, Marc Mußmann, Bernhard M. Fuchs, Rudolf Amann, Ulrich Lass & Marcel M. M. Kuypers, Detoxification of sulphidic African shelf waters by blooming chemolithotrophs, Nature, December 11th, online-early publication, DOI: 10.1038/nature07588

Provided by Max-Planck-Institute for Marine Microbiology