The secret life of frogs

Boyd, along with two postdoctoral researchers, two graduate students and three undergraduates, goes out into the real world—the real world being UNDERC, the University’s environmental research center in Land O’Lakes, Wis.—and observe what frogs are doing in nature.

“Then we create computer models where we explore what might be causing the behavior,” says Boyd, an associate professor in Notre Dame’s Department of Biological Sciences.

Female frogs prefer males with longer calls—a trait linked to better offspring. But how does she choose? The lab’s research is investigating two possible strategies. Does she pick the first male that meets the basic criteria, or—in what Boyd calls the “best of” strategy, make comparisons among several males?

Frogs are useful organisms for conducting basic research, says Boyd, because they bear a considerable resemblance to humans—they have the same basic brain areas, and the same basic behaviors.

“Frogs use vocal communications—as do humans, she explains. “Obviously they have fewer words than we do, and their behaviors are simpler. Because their behaviors are simpler, we’re looking at three behaviors instead of a hundred.”

Another area of research looks at the vocal behavior of bullfrogs, something that’s often a critical component of social interaction. Many behaviors—including vocalization, aggression and reproduction—are influenced by neuropeptides and steroid hormones similar to those found in humans.

“The frog peptide is vasotocin. Humans have a variety called oxytocin. The very same peptide is involved in pair bonding mammals,” Boyd says. “The same chemical is linked to autism.”

An exciting new line of research involves African clawed frogs and a new technique called microarray. The lab is investigating a steroid hormone that’s produced directly in the brain, and trying to understand how it works.

Boyd likens the technique to a glass slide, with probes for each of the 32,000 genes of the frog. A color development system allows researchers to see whether the treatment under investigation makes the genes more or less active—do they produce more or less of their chemical product?

They’ve discovered—for the first time in any organism—that the steroid in question influences two other chemical messengers from the pituitary gland. It’s a potentially important discovery, Boyd says, since the neurochemicals in the brain of a frog are either identical or almost identical to those in the human brain.

“The steroid we’re investigating has been shown to be involved in post-partum depression,” she says. “It has protective effects in Alzheimer’s disease and strokes. I uncover the basic mechanisms that apply across all vertebrates.”

It’s a discovery made possible by advances in nanotechnology, she adds.

“Previously, we did it one gene at a time. I took miniaturization to take it out of the test tube and onto a flat surface.”

In addition to ongoing research, the lab also performs another important role, Boyd adds—training students and post-doctoral researchers to do research, disseminate it to the world, and think critically.

“They learn those things here, so they can work on other important problems” when they begin their own scientific careers, she explains.

Ultimately, the mission of the lab is to advance human, animal and environmental health.

“That’s where our basic research findings have an important role to play,” Boyd says. “You can’t skip this step. We lay the foundation for more applied medical research.”

Provided by University of Notre Dame