Light Scattering Method Reveals Details under Skin

The imaging process involves illuminating a sample with polarized light, which has its electric field oriented in a particular direction, and using a digital camera with a rotating polarization filter to image the light scattered from the sample. Researchers manipulated the polarization to minimize light scattered from the rough skin surface, and positioned the light source in multiple locations to separate out, and delete, light scattered more than one time from deeper sample layers. By using certain polarization settings and combining two images made with the light source in different positions, they generated a processed image that reveals significant subsurface structure.

Polarized light imaging already is used in dermatology to identify the edges of lesions. The new method minimizes the effects of two types of unwanted light scattering at once, and thus, if confirmed by other methods, might someday be used in a clinical setting to produce more detailed images of deeper layers of skin.

The method was developed under a Cooperative Research and Development Agreement between the two institutions. The project adapted light scattering techniques originally developed by NIST researchers to image surface and subsurface features in inorganic materials such as silicon wafers, mirrors and paint coatings. Scientists currently are working on making the new method easier and faster to use.

*Publication: J.C. Ramella-Roman, D. Duncan, T.A. Germer. 2005. Out-of-plane polarimetric imaging of skin: Surface and subsurface effects. In Photonic Therapeutics and Diagnostics, Nikiforos Kollias et al., eds. Proc. SPIE 5686 (forthcoming).