New single-molecule imaging system ends pRNA debate over phi29 motor

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A dual view graph generated by the imaging system shows fluorescence intensity vs. time for two types of pRNA. Each step down represents the photobleaching or loss of one marker. Each step on the final graph represents one single molecule. The insert ...
A dual view graph generated by the imaging system shows fluorescence intensity vs. time for two types of pRNA. Each step down represents the photobleaching, or loss, of one marker. Each step on the final graph represents one single molecule. The insert illustrates the phi29 motor ring of six pRNA. (Purdue graphic/Guo Laboratories)

Scientists are able to view active molecules within a biological motor of the nanometer scale with the help of a new imaging system far more sensitive and powerful than existing optical microscopes.


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