Going Live With Click Chemistry

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These time-lapse images of a single cell over a one hour period were recorded using the copper-free click chemistry reaction of azides and DIFO developed by Bertozzi et. al. The arrow points to the azide probe.
These time-lapse images of a single cell over a one hour period were recorded using the copper-free click chemistry reaction of azides and DIFO developed by Bertozzi, et. al. The arrow points to the azide probe.
Click chemistry, one of the most exciting and proficient new techniques for labeling biomolecules in vitro, has now been extended to studies in the context of live cells as well. This breakthrough opens the door for applications to live cell imaging of numerous biomolecules, including glycans, proteins and lipids. The new version of click chemistry was developed by researchers with the Lawrence Berkeley National Laboratory and the University of California at Berkeley.


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